The pxlence assays allowed us to replace Sanger sequencing with NGS for all our sequencing-based diagnostic tests in an ISO15189-accredited environment. The major advantages of the pxlence assays are a high success rate and uniform PCR conditions, enabling PCR automatisation. Moreover, the availability of assays for the entire human exome allows easy and fast expansion of our diagnostic portfolio (De Leeneer et al. 2015).

We use the pxlence assays for both Sanger sequencing and MPS validation. In the past, we designed primers ourselves. We now prefer pxlence assays as they are easy-to-use, less time-consuming and work with a universal PCR protocol. We will not hesitate to work with pxlence for other applications in the future if the opportunity arises.

At Amplexa Genetics we have been using pxlence assays for some time now and we are very satisfied with the solution. It is saving us valuable time at no extra cost. Prior to pxlence we spent a lot of time on designing primers and subsequent testing and optimizing the amplicons. Now we just order the pre-designed and ready to use amplicons from pxlence and run them at the defined standard conditions - works every time. Adds no extra cost but saves us a lot of time!