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Universal detection of DNA using RainbowTM probes for digital PCR and qPCR
With patent-pending RainbowTM nucleic acid detection probes, no more need to design and optimize a target-specific probe. We modify one of the primers of an existing pair (converting it into a RainbowTM primer) so that it is recognized by a RainbowTM probe during amplicon generation.
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With one specific RainbowTM probe for each detection channel, you can quantify any target in a multiplex reaction. Ideally suited for applications that require many different probes (e.g. (higher-order) multiplexing) or applications that only need small amounts of probes (e.g. pilot experiments or patient-specific assays).
Principle
Advantages
No time lost designing and optimizing a probe sequence
Available on stock with quick delivery
Maximally cost-efficient: same probe for different targets, no more unused stock
Key features
Developed for digital PCR quantification (high signal to noise)
Highly concentrated (60X) to enable multiplexing
Ultrashort amplicons possible (no space needed for a probe)
Ideal for quantification on FFPE tissue and liquid biopsies
Order
Intended use
RainbowTM probe
RBO-01-B-FAM
ddPCR
65 €
RainbowTM primer
10,000 rxns
25 €
As a reference, 0.25 nmol RainbowTM probe is sufficient for 100 reactions of 20 µL with 125 nM probe concentration.
Detailed ordering instructions are mentioned in the Instructions for use (section 'Probe and primer order workflow').
Detailed ordering instructions are mentioned in the Instructions for use (section 'Probe and primer order workflow').
Overview of 13 RainbowTM probes for any detection channel of any dPCR system
Probe name
Fluorophore
Compatible primer
Compatible instruments
RBO-01-A-425
ATTO 425
tag A
Digital LightCycler, DQ24
RBO-01-B-FAM
FAM
tag B
QX200, QX ONE, QX600, QIAcuity, naica 3, naica 6, Nio, Digital LightCycler, DQ24, QuantStudio Absolute Q
RBO-01-C-YAK
Yakima Yellow
tag C
naica 6, Nio
RBO-01-C-HEX
HEX
tag C
QX200, QX ONE, QX600, QIAcuity, naica 3, Digital LightCycler, DQ24, QuantStudio Absolute Q
RBO-01-E-482
DY-482XL
tag E
QIAcuity
RBO-01-A-550
ATTO 550
tag A
QIAcuity, naica 6, Nio, QuantStudio Absolute Q
RBO-01-D-TEX
Texas Red
tag D
QIAcuity, Digital LightCycler
RBO-01-D-ROX
ROX
tag D
QX600, QIAcuity, naica 6, Nio, DQ24
RBO-01-A-590
ATTO 590
tag A
QX600
RBO-01-E-521
DY-521XL
tag E
Nio
RBO-01-F-CY5
Cy5
tag F
QX ONE, QX600, QIAcuity, naica 3, naica 6, Nio, Digital LightCycler, DQ24, QuantStudio Absolute Q
RBO-01-G-C55
Cy5.5
tag G
QX ONE, QX600, Digital LightCycler, DQ24
RBO-01-G-700
ATTO 700
tag G
naica 6, Nio
Please contact us if you need other fluorophores.
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