pxlence - Rainbow probes
Universal detection of nucleic acids using RainbowTM primers and probes for digital PCR
With patent-pending RainbowTM nucleic acid detection probes, no more need to design and optimize a target-specific probe. We modify one of the oligonucleotides of your existing PCR primer pair (converting it into a RainbowTM primer) so that it is recognized by a RainbowTM probe during amplicon generation.
With 2 to 6 RainbowTM probes (depending on your digital PCR instrument), you can quantify any target in multiplex. Ideally suited for applications that require many different probes (e.g. (higher-order) multiplexing) or applications that only need small amounts of probes (e.g. pilot experiments, patient-specific assays).
Principle
 
 
Advantages
No time lost designing and optimizing a probe sequence
Available on stock with quick delivery
Maximally cost efficient: same probe for different targets, no more unused stock
Key features
Developed for digital PCR quantification (high signal to noise)
Highly concentrated (60X) to enable multiplexing
Ultrashort amplicons possible (no space needed for a probe)
Ideal for quantification on FFPE tissue and liquid biopsies
Order
 
RainbowTM probe
PXL-RBO-001-P-FAM
 
65 €
 
RainbowTM primer
10,000 rxns
 
 
15 €
 
Reactions are assumed 20 µL volume with 125 nm RainbowTM probe and 100 nM RainbowTM primer.
Detailed ordering instructions are mentioned in the Instructions for use (section 'Probe and primer order workflow').
Overview of 11 RainbowTM probes for 6 different detection channels compatible with virtually all dPCR systems
 
Catalog number
Fluorophore
Probe channel
Compatible instruments
PXL-RBO-001-P-FAM
FAM
purple (P)
QX200, QX ONE, QX600, QIAcuity, naica 3, naica 6, Nio+, Digital LightCycler, DQ24, QuantStudio Absolute Q
PXL-RBO-001-B-YY
Yakima Yellow
blue (B)
naica 6, Nio+
PXL-RBO-001-B-HEX
HEX
blue (B)
QX200, QX ONE, QX600, QIAcuity, naica 3, Digital LightCycler, DQ24, QuantStudio Absolute Q
PXL-RBO-001-G-ATTO425
ATTO425
green (G)
Digital LightCycler, DQ24
PXL-RBO-001-G-ATTO550
ATTO550
green (G)
QIAcuity, naica 6, Nio+
PXL-RBO-001-G-ATTO590
ATTO590
green (G)
QX600
PXL-RBO-001-Y-TR
Texas Red
yellow (Y)
QIAcuity, Digital LightCycler
PXL-RBO-001-Y-ROX
ROX
yellow (Y)
QX600, QIAcuity, naica 6, Nio+, DQ24, QuantStudio Absolute Q
PXL-RBO-001-O-CY5
Cy5
orange (O)
QX ONE, QX600, QIAcuity, naica 3, naica 6, Nio+, Digital LightCycler, DQ24, QuantStudio Absolute Q
PXL-RBO-001-R-CY55
Cy5.5
red (R)
QX ONE, QX600, Digital LightCycler, DQ24
PXL-RBO-001-R-ATTO700
ATTO700
red (R)
naica 6, Nio+
 

Please contact us if you need other fluorophores.
Downloads
Instructions for use