With patent-pending RainbowTM nucleic acid detection probes, no more need to design and optimize a target-specific probe. We modify one of the oligonucleotides of your existing PCR primer pair (converting it into a RainbowTM primer) so that it is recognized by a RainbowTM probe during amplicon generation.
With 2 to 6 RainbowTM probes (depending on your digital PCR instrument), you can quantify any target in multiplex. Ideally suited for applications that require many different probes (e.g. (higher-order) multiplexing) or applications that only need small amounts of probes (e.g. pilot experiments, patient-specific assays).
No time lost designing and optimizing a probe sequence
Available on stock with quick delivery
Maximally cost efficient: same probe for different targets, no more unused stock
Developed for digital PCR quantification (high signal to noise)
Highly concentrated (60X) to enable multiplexing
Ultrashort amplicons possible (no space needed for a probe)
Ideal for quantification on FFPE tissue and liquid biopsies
Reactions are assumed 20 µL volume with 125 nm Rainbow
TM probe and 100 nM Rainbow
TM primer.
Detailed ordering instructions are mentioned in the
Instructions for use (section 'Probe and primer order workflow').
Overview of 11 RainbowTM probes for 6 different detection channels compatible with virtually all dPCR systems
Catalog number
Fluorophore
Probe annotation
Compatible instruments
PXL-RBO-001-P-FAM
FAM
purple (P)
QX200, QX ONE, QX600, QIAcuity, naica 3, naica 6, Nio, Digital LightCycler, DQ24, QuantStudio Absolute Q
PXL-RBO-001-B-YY
Yakima Yellow
blue (B)
naica 6, Nio
PXL-RBO-001-B-HEX
HEX
blue (B)
QX200, QX ONE, QX600, QIAcuity, naica 3, Digital LightCycler, DQ24, QuantStudio Absolute Q
PXL-RBO-001-G-ATTO425
ATTO 425
green (G)
Digital LightCycler, DQ24
PXL-RBO-001-G-ATTO550
ATTO 550
green (G)
QIAcuity, naica 6, Nio, QuantStudio Absolute Q
PXL-RBO-001-G-ATTO590
ATTO 590
green (G)
QX600
PXL-RBO-001-Y-TR
Texas Red
yellow (Y)
QIAcuity, Digital LightCycler
PXL-RBO-001-Y-ROX
ROX
yellow (Y)
QX600, QIAcuity, naica 6, Nio, DQ24
PXL-RBO-001-O-CY5
Cy5
orange (O)
QX ONE, QX600, QIAcuity, naica 3, naica 6, Nio, Digital LightCycler, DQ24, QuantStudio Absolute Q
PXL-RBO-001-R-CY55
Cy5.5
red (R)
QX ONE, QX600, Digital LightCycler, DQ24
PXL-RBO-001-R-ATTO700
ATTO 700
red (R)
naica 6, Nio
Please contact
us if you need other fluorophores.