Universal detection of nucleic acids using RainbowTM primers and probes for digital PCR
With RainbowTM nucleic acid detection probes, no more need to design and optimize a probe. We modify one of your oligonucleotides of an existing PCR primer pair (converting it into a RainbowTM primer) to be recognized by a RainbowTM probe during amplicon generation.
With 2 to 6 RainbowTM probes (depending on your digital PCR instrument), you can quantify any target in multiplex. Ideally suited for applications that require many probes (e.g. (higher-order) multiplexing) or applications that only need small amounts of probes (e.g. pilot experiments, personalized assays).
No time lost designing and optimizing a probe sequence
Available on stock with quick delivery
Maximally cost efficient: same probe for different targets, no more unused stock
Developed for digital PCR quantification (high signal to noise)
Highly concentrated (60X) to enable multiplexing
Ultrashort amplicons possible (no space needed for a probe)
Ideal for quantification on FFPE tissue and liquid biopsies
Reactions are assumed 20 µL volume with 125 nm RainbowTM probe and 100 nM RainbowTM primer. Detailed ordering instructions are mentioned in the Instructions for use (section 'Probe and primer order workflow').
Overview of 11 RainbowTM probes for 6 different detection channels compatible with virtually all dPCR systems