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ConfirmPCRTM assays for amplicon sequencing
We have 1 million best-in-class PCR primer pairs for targeted resequencing of all human exons. Multilayered in silico verification and extensive wet-lab validation of more than 10,000 assays for 500 disease genes guarantee success. We return the money if the results are not satisfactory.
The ConfirmPCRTM assays are perfect for variant confirmation of exome and whole genome sequencing results, and for pedigree analysis of variants of unknown significance.
We offer short and long ConfirmPCRTM assays, supporting Sanger and massively parallel (next generation) sequencing of DNA from fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tissue or blood.
Use the search form below to find the ConfirmPCRTM assay(s) for your region of interest, ready to order in the next step.
The ConfirmPCRTM assays are perfect for variant confirmation of exome and whole genome sequencing results, and for pedigree analysis of variants of unknown significance.
We offer short and long ConfirmPCRTM assays, supporting Sanger and massively parallel (next generation) sequencing of DNA from fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tissue or blood.
Use the search form below to find the ConfirmPCRTM assay(s) for your region of interest, ready to order in the next step.
On-target
Our ConfirmPCRTM primer assays are highly specific by design. We model the impact of mismatches in the primer annealing sites on PCR selectivity and efficiency to provide best-in-class assays.
Fast and accurate
We guarantee short delivery times and accurate results from 1st attempt. Our thorough in silico validation checks combined with a high-quality oligonucleotide synthesis process ensures our customers maximal PCR success rate.
Every exonic sequence
ConfirmPCRTM assays cover more than 99% of the human exome, and include sufficient intronic sequence to maximize Sanger sequencing-based exonic variant confirmation. The PCR assays are compatible with high-quality DNA and fragmented DNA (e.g. from FFPE tissue).