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Human Sample ID Kit
The Human Sample ID Kit is an easy sequencing-based genotyping assay that targets polymorphic SNPs and gender markers, creating a highly specific intrinsic genetic label for each DNA sample. It comes in 2 versions, with either 50 or 13 markers. In a single reaction, sequencing-ready libraries are generated compatible with Illumina sequencing platforms. The Human Sample ID Kit is designed to be complementary with established enrichment platforms and has been validated for both high-quality genomic, low-quality formalin-fixed paraffin-embedded (FFPE) and cell-free DNA templates.
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Advantages
Superior coverage uniformity
Easy integration in WES and WGS
Single reaction simple workflow
Analysis software and standardized report
Key features
Very high discrimination power >1:10 quadrillion (<1E-16)
Ambient shipment
Maximum compatibility with established enrichment platforms
Single-use Unique Dual Index plates for maximum convenience
Products
Highlights for Human Sample ID Kit
Sample type
blood DNA, FFPE tissue DNA, plasma cfDNA
Input requirements
2 - 20 ng
Total time
3 hours
Hands-on time
20 minutes
Number of markers
50 (incl. 6 gender markers)
Very high discrimination power
between 1.36E-17 and 7.29E-18 depending on population
On-target specificity
> 90 %
Coverage uniformity, above 20 % of the mean
100 %
Compatible platform
Illumina
Number of samples per million read pairs
> 96 (at 30X coverage)
Workflow
The protocol consists of a single-tube reaction in which multiplex target amplification of polymorphic markers is combined with proprietary sample indexing to generate sequencing-ready libraries. All libraries are then equivolumetrically pooled, followed by simple clean-up, quantification, and sequencing.
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