Our Human Sample ID Kit is an easy sequencing-based genotyping assay that targets 44 polymorphic SNPs and 6 gender markers, creating a highly specific intrinsic genetic label for each patient sample. In just one simple PCR step, sequencing-ready libraries are generated compatible with Illumina sequencing platforms. The Human Sample ID multiplex panel is designed to be complementary with established enrichment platforms and has been validated for both high-quality genomic, low-quality formalin-fixed paraffin-embedded (FFPE) and cell-free DNA templates.
Superior coverage uniformity
Easy integration in WES and WGS
Very high discrimination power >1:10 quadrillion (<1E-16)
Maximum compatibility with established enrichment platforms
Human Sample ID Kit
PXL-SID-001
96 reactions
Human Sample ID Index Set
PXL-IND-001
96 reactions
12 x 8
Human Sample ID Index Set
Sample type
blood DNA, FFPE tissue DNA, plasma cfDNA
Input requirements
1 - 20 ng
Number of assays
50 (incl. 6 gender markers)
Very high discrimination power
between 1.36E-17 and 7.29E-18 depending on population
On-target specificity
> 90 %
Coverage uniformity, above 20 % of the mean
100 %
Compatible platform
Illumina
Number of samples per million read pairs
192 (at 30X coverage)
The protocol consists of a single-tube reaction in which multiplex target amplification of 50 markers is combined with sample indexing to generate sequencing-ready libraries. All libraries are then equivolumetrically pooled, followed by simple clean-up, quantification and sequencing.