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Human Sample ID Kit
Our Human Sample ID Kit is an easy sequencing-based genotyping assay that targets 44 polymorphic SNPs and 6 gender markers, creating a highly specific intrinsic genetic label for each patient sample. In just one simple PCR step, sequencing-ready libraries are generated compatible with Illumina sequencing platforms. The Human Sample ID multiplex panel is designed to be complementary with established enrichment platforms and has been validated for both high-quality genomic, low-quality formalin-fixed paraffin-embedded (FFPE) and cell-free DNA templates.
Advantages
Superior coverage uniformity
Easy integration in WES and WGS
Simple workflow
Key features
Very high discrimination power >1:10 quadrillion (<1E-16)
Ambient shipment
Maximum compatibility with established enrichment platforms
Content
Highlights
Sample type
blood DNA, FFPE tissue DNA, plasma cfDNA
Input requirements
1 - 20 ng
Total time
3 hours
Hands-on time
20 minutes
Number of assays
50 (incl. 6 gender markers)
Very high discrimination power
between 1.36E-17 and 7.29E-18 depending on population
On-target specificity
> 90 %
Coverage uniformity, above 20 % of the mean
100 %
Compatible platform
Illumina
Number of samples per million read pairs
192 (at 30X coverage)
Workflow
The protocol consists of a single-tube reaction in which multiplex target amplification of 50 markers is combined with sample indexing to generate sequencing-ready libraries. All libraries are then equivolumetrically pooled, followed by simple clean-up, quantification and sequencing.
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