pxlence's Human Sample ID Kit is an easy and flexible multiplex genotyping assay that targets 44 polymorphic SNPs and 6 gender markers, creating a highly specific intrinsic genetic label for each sample. In just two simple PCR steps sequencing-ready libraries are generated compatible with Illumina sequencing platforms. The Human Sample ID multiplex panel is designed to be complementary with established enrichment platforms and has been validated for both high-quality genomic, low-quality Formalin-Fixed Paraffin-Embedded (FFPE) and cell-free DNA templates.
Easy integration in WES and WGS
Very high discrimination power >1:10,000,000,000,000,000 (<1E-16)
Maximum compatibility with established enrichment platforms
gDNA, FFPE, cfDNA
1 - 20 ng
Number of assays
50 (incl. 6 gender markers)
Very high discrimination power
between 1.36E-17 and 7.29E-18 depending on population
> 90 %
Coverage uniformity, above 20 % of the mean
Average number of samples on a MiSeq Reagent
153 (at minimal 100X coverage)
Kit v2 Nano run (2 x 150 bp)
This protocol consists of two PCR steps : a multiplex PCR step for the simultaneous amplification of 50 targets and an indexing step for the addition of dual indexed adapters. After a simple clean-up to remove residual oligonucleotides, the library is ready-to-be sequenced.