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Human Sample ID Kit
The Human Sample ID Kit is an easy-to-use sequencing-based genotyping assay that targets polymorphic SNPs and gender markers, creating a highly specific intrinsic genetic label for each DNA sample. It comes in 2 versions, with either 49 or 13 markers. In a single reaction, sequencing-ready libraries are generated compatible with Illumina sequencing platforms. The Human Sample ID Kit is designed to be compatible with popular exome enrichment platforms and has been validated for both high-quality genomic, low-quality formalin-fixed paraffin-embedded (FFPE) and cell-free DNA templates.
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Advantages
Excellent coverage uniformity
Easy integration in WES and WGS
Single reaction workflow
Analysis software with report
Key features
Extremely low random probability match of 1 in 27 quadrillion individuals
Ambient temperature shipment
Maximum compatibility with exome enrichment kits
Convenient single-use Unique Dual Index plates
Products
Highlights for Human Sample ID Kit
Sample type
blood DNA, FFPE tissue DNA, plasma cfDNA
Input requirements
2 - 20 ng
Total time
3 hours
Hands-on time
20 minutes
Number of markers
49 (incl. 6 gender markers)
Random match probability
between 3.68E-17 and 4.23E-18 depending on population
On-target specificity
> 90 %
Coverage uniformity, within 2-fold of the median
98 %
Compatible platform
Illumina
Number of samples per million read pairs
> 96 (at 30X coverage)
Workflow
The protocol consists of a single-tube reaction in which multiplex target amplification of polymorphic markers is combined with proprietary sample indexing to generate sequencing-ready libraries. All libraries are then equivolumetrically pooled, followed by clean-up, quantification, and sequencing. Software is available for analysis and reporting.
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