pxlence is a Ghent University spin-off company providing best-in-class PCR assays for targeted amplification and sequencing of the human exome. pxlence's flagship product is an online catalogue of almost one million predesigned PCR assays for targeted resequencing of all canonical exons of human protein coding genes. Assays are used for Sanger sequencing based confirmation of variants and for massively parallel sequencing based identification of new variants.
Advantages & key features
Uniform PCR conditions
High success rate, 99.9% coverage of the human exome
Uniform sequence coverage (read depth)
Proven to work in several applications (knowledge center), including clinical molecular diagnostics
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Applications
Fast confirmation of NGS findings derived from gene panels,
whole exome sequencing and others
Targeted resequencing of custom gene panels
Targeted resequencing of a custom selection of disease-causing mutations
Cell line authentication and patient sample tracking
Much more ... design your own experiment
News
2016-02-16
pxlence will be present at the European Society for Human Genetics in Barcelona, May 21-24, 2016. Contact us if you would like to setup a meeting to discuss your targeted resequencing needs.
About
pxlence is a Ghent University spin-off company founded in 2015 by dr. Steve Lefever, dr. Frauke Coppieters and prof. Jo Vandesompele with an exclusive commercial license for PCR based target enrichment technology from UGent. Using the state-of-the-art high-throughput primer design software pipeline primerXL, almost one million assays for amplification of DNA from both fresh frozen and formalin fixed parafin embedded (FFPE) material covering over 99% of the human exome were expertly designed. These best-in-class assays enable the enrichment of any exonic region of interest for targeted resequencing or for confirmation of variants by means of Sanger or next-generation sequencing. Approximately 3,000 individual assays for 200 genes have been tested in a diagnostic setting (ISO 15189) with a wet-lab success rate exceeding 95%. Our customers include academic research centers, hospitals, clinical diagnotics labs and industrial players in the field of PCR based target enrichment.
Our company name pxlence (pronounced 'pixellence') refers to
primerXL : the in house developed primer design pipeline used to create our assays
pixels : we see the image of the genome as our playground, we are able to target any pixel you wish, either using our ready-to-go assays for the human exome or by designing custom assays for any region of interest
excellence : we strive for excellent assay performance at a single PCR condition
Knowledge center
Below, you can find peer-reviewed articles using pxlence gene panels, illustrating the use of different high-throughput PCR and sequencing platforms :
Here, you can find more articles, presentations and brochures.
* discount only applicable when purchasing 3 or more assays, discount can only be used once per laboratory, can not be cummulated with other discounts